Signalling

Part:BBa_K4380001:Design

Designed by: Brigita Duchovska   Group: iGEM22_Vilnius-Lithuania   (2022-09-30)


OmpA signalling sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

An additional ATG is needed at the start of the sequence. The amino acid sequence of the signal peptide was deduced from the DNA sequence as follows: Met-Lys-Lys-Thr-Ala-Ile-Ala-Ile-Ala-Val-Ala-Leu-Ala-Gly-Phe-Ala-Thr-Val-Ala-Gin-Ala.

Source

The original part comes from an article by Movva, N. R., Nakamura, K., & Inouye, M., 1980 [1].The amino acid sequence complies with all the features of the loop model proposed for the mechanism of protein secretion across the cytoplasmic membrane..

References

[1] Movva, N. R., Nakamura, K., & Inouye, M. (1980). Amino acid sequence of the signal peptide of ompA protein, a major outer membrane protein of Escherichia coli. The Journal of Biological Chemistry, 255(1), 27–29.